세균의 활성산소종과 활성질소종에 대한 내성에서 NADH dehydrogenase-2 결손의 효과
Effect of NADH dehydrogenase-2 Deficiency to the Bacterial Resistance against Reactive Oxygen/Nitrogen Species
박희정, 채권석, 방일수,
소속 상세정보
박희정 ( Park Hee-Jeong ) - 조선대학교 치과대학 구강미생물학 및 면역학교실
채권석 ( Chae Kwon-Seok ) - 경북대학교 사범대학 과학교육학부
방일수 ( Bang Iel-Soo ) - 조선대학교 치과대학 구강미생물학 및 면역학교실
KMID : 0829220100340060347
Abstract
The electron transport chain (ETC) delivers electrons from many substrates to reduce molecular oxygen to water. ETC accomplishes the stepwise transfer of electrons through series of protein complexes conferring oxidation-reduction reactions with concomitant transport of proton across membrane, generating a proton gradient which leads ATP synthesis by F0F1ATPase. Bacterial ETC initiates with oxidation of NADH by NADH dehydrogenase complex (complex Ⅰ). Therefore, damage of complex Ⅰ leads to insufficient function of ETC and accumulation of NADH inside the cell.
Contribution of ETC activity and its consequent changes of NADH levels to bacterial damage response against reactive oxygen and nitrogen species (ROS/RNS) has been poorly understood. In this study, by constructing ndh mutant Salmonella lacking complex Ⅰ NADH dehydrogenase 2, we evaluated the effect of ETC deficiency to bacterial resistance against ROS and RNS. The growth of ndh mutant Salmonella is impaired in the culture media containing hydrogen peroxide, but rather accelerates in the media containing nitric oxide donors. Data suggest that redox potential of NADH accumulated inside the cell by ETC blockage may affect inversely to bacterial resistance against reactive oxygen species and reactive nitrogen species.
키워드
Electron transport chain; NADH dehydrogenase-2; Salmonella; Reactive oxygen/nitrogen species
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